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Sie suchten nach: Proteinextraktions-Reagenzien

Efficient cell lysis and maximum protein extraction yields are vital to high-quality recombinant protein purification. The protease inhibitor cocktails are for lysing mammalian cells, buffers that solubilize plasma and intracellular membranes, break weak intermolecular bonds, and solubilize most proteins. Making procedures easier to perform, these protein extraction reagents can also be used to isolate proteins from both the cytoplasmic and nuclear fractions of cultured cells.

Efficient cell lysis and maximum protein extraction yields are vital to high-quality recombinant protein purification. The protease inhibitor cocktails are for lysing mammalian cells, buffers that solubilize plasma and intracellular membranes, break weak intermolecular bonds, and solubilize most proteins. Making procedures easier to perform, these protein extraction reagents can also be used to isolate proteins from both the cytoplasmic and nuclear fractions of cultured cells.


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Antigen retrieval reagent, IHC

Lieferant: ENZO LIFE SCIENCES
Beschreibung: Antigen retrieval reagent is used to recover antigens masked by fixation in cross linking fixatives such as formalin.

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Product Image-786-793

Immobilised Ficin

Artikel-Nr: (786-793)
Lieferant: G-Biosciences
Beschreibung: Ficin (or Ficain) is a cysteine protease enzyme (EC 3.4.22.3) isolated from fig latex is that has the endopeptidase activity to cleave Immunoglobulin G (IgG) molecules in the hinge region.
VE: 1 * 5 mL
Product Image-786-598

Biotin resin

Artikel-Nr: (786-598)
Lieferant: G-Biosciences
Beschreibung: Immobilised biotin resin is designed for the high affinity chromatography purifications of avidin, streptavidin and neutravidin protein. The resin consists of biotin coupled to 6% cross-linked agarose.
VE: 1 * 5 mL
Product Image-DGA01

Optimizer blueBALLS™

Artikel-Nr: (DGA01)
Lieferant: G-Biosciences
Beschreibung: The 'critical micelle concentration' (CMC) of a detergent varies with temperature, pH, ionic strength, detergent concentration, purity and presence of organic agents in the detergent. Using a large excess of detergent may pose problems during purification procedures or other downstream applications.
VE: 1 * 500 ST
Product Image-786-244

SilverOUT™, G-Biosciences

Artikel-Nr: (786-244)
Lieferant: G-Biosciences
Beschreibung: SilverOUT removes interfering silver ions from gel pieces.
VE: 1 * 100 Tests
Product Image-786-681

InGel™ Blue for protein digestion

Artikel-Nr: (786-681)
Lieferant: G-Biosciences
Beschreibung: InGel™ Blue provides a complete set of reagents for the in-gel tryptic digestion and extraction of peptides for mass spectrometry (MALDI and LC MS/MS). The kit is specifically designed for use with Coomassie® or fluorescent stained protein spots/bands.
VE: 1 * 1 KIT
Product Image-786-241

InGel™ for protein digestion

Artikel-Nr: (786-241)
Lieferant: G-Biosciences
Beschreibung: InGel™ provides a reliable method for the proteolytic digestion and subsequent extration of silver stained proteins in gel for subsequent analysis by mass spectrometry.
VE: 1 * 1 KIT
Product Image-786-077

Protein reducing agents

Lieferant: G-Biosciences
Beschreibung: Protein-S-S-Reductant™ uses TCEP (Tris [2-carboxyethyl] phosphine), a popular alternative to β-mercaptoethanol and DTT (dithiothreitol). TCEP improves stability, increases effectiveness, and reduces proteins over a wider range of pH, including lower acidic pHs. Protein-S-S-Reductant™ completely reduces stable disulfide bonds in less than 5 minutes at room temperature and is compatible with the protein alkylation reactions. This ready to use solution is at a neutral pH and stabilised for long-term storage. Simply supplement Protein-S-S-Reductant™ in place of DTT or β-mercaptoethanol and boil the sample.

Product Image-PIER88701

Universal nuclease for cell lysis, Pierce™

Lieferant: Thermo Fisher Scientific
Beschreibung: The Pierce™ Universal Nuclease for Cell Lysis is ideal for a wide variety of applications where complete digestion of nucleic acids is needed when preparing cell lysates.

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Fluor-de-Lys® Developer II concentrate

Artikel-Nr: (ENZOBMLKI1761250)
Lieferant: ENZO LIFE SCIENCES
Beschreibung: FLUOR DE LYS® Developer II is an essential reagent for signal generation in assays done with the FLUOR DE LYS®-HDAC8, FLUOR DE LYS®-SIRT1 and FLUOR DE LYS®-SIRT2 substrates. Deacetylation of the FLUOR DE LYS® substrate sensitises it so that, in a second step, treatment with the FLUOR DE LYS® Developer II produces a fluorophore. Typically, an ‘inhibitor stop’ will be included in the 1X Developer dilution (e.g. nicotinamide for sirtuins).
VE: 1 * 1,25 mL

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Product Image-PIER90054

Endoproteinase Glu-C protease, MS grade, Pierce™

Artikel-Nr: (PIER90054)
Lieferant: Thermo Fisher Scientific
Beschreibung: Glu-C protease is a highly specific endoproteinase use to improve sequence coverage in mass spectrometry protein identification applications.
VE: 1 * 50 µG

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Product Image-PIER90056

Endoproteinase chymotrypsin protease (TLCK treated), MS grade, Pierce™

Artikel-Nr: (PIER90056)
Lieferant: Thermo Fisher Scientific
Beschreibung: Chymotrypsin protease is a purified native endoproteinase validated for maximum activity and selectivity in proteomic applications.
VE: 1 * 100 µG
Product Image-PIER90053

Endoproteinase Asp-N protease, MS grade, Pierce™

Artikel-Nr: (PIER90053)
Lieferant: Thermo Fisher Scientific
Beschreibung: Asp-N protease is a highly specific endoproteinase used to improve sequence coverage in mass spectrometry protein identification applications.
VE: 1 * 2 µG

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Product Image-786-599

Immobilised Iminobiotin Resin

Artikel-Nr: (786-599)
Lieferant: G-Biosciences
Beschreibung: Immobilised Iminobiotin Resin consists of iminobiotin, a cyclic guanido analog of biotin, covalently coupled to 6% crosslinked agarose. The resin allows for the purification of avidin, streptavidin and Neutravidin and their subsequent gentle elution using non denaturing elution buffers.
VE: 1 * 5 mL
Product Image-786-087

Carrier proteins

Lieferant: G-Biosciences
Beschreibung: The use of peptides for the generation of antibodies against specific peptides has become an essential tool in proteomic research. A crucial step in using peptides as antigens is their coupling to carrier proteins. Peptides are designed to be good epitopes by themselves; however they fail to elicit a strong antibody response. The reason for this is that the epitopes are able to bind to the B-cells, but are too small to contain additional motifs required to associate with class II molecules and T-cell receptors. The carrier proteins, once coupled to the peptides, provide these additional motifs and generate a strong antibody response. All three carrier proteins are also offered as activated carrier proteins, named as ActiveHOOK™.

Image Unavailable-786-214

Detergent removal system, DetergentOUT™ Tween®

Lieferant: G-Biosciences
Beschreibung: DetergentOUT™ Tween® is a spin column format detergent removal resin for polysorbate or Tween® detergents or surfactants.

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