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Image Unavailable-PIERA35393

DSP (Dithiobis(succinimidyl propionate)), No-Weigh™ Format, Pierce™

Artikel-Nr: (PIERA35393)
Lieferant: Thermo Fisher Scientific
Beschreibung: Thermo Scientific Pierce DSP (Lomant's Reagent) is a water-insoluble, homo-bifunctional N-hydroxysuccimide ester (NHS ester) crosslinker that is thiol-cleavable, primary amine-reactive, and useful for many applications. DSP contains an amine-reactive NHS ester at each end of an 8-carbon spacer arm. NHS esters react with primary amines at pH 7–9 to form stable amide bonds and releasing N-hydroxy-succinimide. Proteins, including antibodies, generally have several primary amines in the side chain of lysine (K) residues and the N-terminus of each polypeptide that are available as targets for NHS ester crosslinking reagents. DSP is non-sulfonated and insoluble in water, so it must first be dissolved in an organic solvent and then added to the aqueous reaction mixture. Because DSP does not possess a charged group, it is lipophilic and membrane-permeable and so useful for intracellular and intramembrane conjugation. A sulfonated analog of DSP (DTTSP) is water soluble. DSS, the non-cleavable analog of the DSP crosslinker is also available for applications that require a stable spacer arm that cannot be cleaved in the presence of reducing agents.
VE: 1 * 10 mg
Product Image-PIER22113

Pierce™ PEGylated SMCC Crosslinkers, Heterobifunctional

Lieferant: Thermo Fisher Scientific
Beschreibung: Cross linkers are used for the identification of near-neighbour protein relationships and ligand-receptor interactions. Homobifunctional, amine-reactive NHS esters or imidates, and heterobifunctional, amine-reactive, photoactivatable phenyl azides are the most commonly used crosslinkers for these applications.

Product Image-PIER26175

Sulpho-SDAD (Sulpho-NHS-SS-Diazirine) (Sulphosuccinimidyl 2-[(4,4′-azipentanamido)ethyl]-1,3′-dithiopropionate], Pierce™

Artikel-Nr: (PIER26175)
Lieferant: Thermo Fisher Scientific
Beschreibung: Thermo Scientific Pierce Sulfo-SDAD (Sulfo-NHS-SS-Diazirine) combines proven NHS-ester and diazirine-based photoreaction chemistries with conjugate amine-containing molecules with nearly any other functional group via long-wave UV-light activation. A 13.5Å spacer arm containing a cleavable disulfide bond separates the two photoreactive groups.
VE: 1 * 50 mg
Product Image-PIER46402

Dyes, DyLight™

Lieferant: Thermo Fisher Scientific
Beschreibung: DyLight™ Dyes have absorption spectra ranging from 350 nm to 770 nm and match the output wavelengths of common fluorescence instrumentation.

Image Unavailable-PIER20593

DSG (Disuccinimidyl glutarate), Pierce™

Lieferant: Thermo Fisher Scientific
Beschreibung: Thermo Scientific Pierce DSG is a water-insoluble, homo-bifunctional N-hydroxysuccinimide ester (NHS-ester) crosslinker often used for conjugating radiolabeled ligands to cell-surface receptors. The NHS ester is the simplest and most commonly used reactive group for crosslinking and labeling proteins and peptides. NHS esters react with primary amines on the N-termini of peptides and the  amine of lysine residues, forming a stable, covalent amide bond and releasing the NHS group.

Image Unavailable-PIERA35394

SMCC (N-Succinimidyl 4-(N-Maleimidomethyl)cyclohexanecarboxylate), No-Weigh™ Format, Pierce™

Artikel-Nr: (PIERA35394)
Lieferant: Thermo Fisher Scientific
Beschreibung: Thermo Scientific Pierce SMCC is a hetero-bifunctional crosslinker that contain N-hydroxysuccinimide (NHS) ester and maleimide groups that allow covalent conjugation of amine- and sulfhydryl-containing molecules. NHS esters react with primary amines at pH 7–9 to form amide bonds, while maleimides react with sulfhydryl groups at pH 6.5–7.5 to form stable thioether bonds. In aqueous solutions, NHS ester hydrolytic degradation is a competing reaction whose rate increases with pH. The maleimide group is more stable than the NHS-ester group, but will slowly hydrolyze and lose its reaction specificity for sulfhydryls at pH values > 7.5. For these reasons, conjugations with these crosslinkers are usually performed at pH 7.2–7.5, with the NHS ester (amine-targeted) reacted before or simultaneous with the maleimide (sulfhydryl-targeted) reaction.
VE: 1 * 10 mg
Product Image-ACRO380870050

Benzyl propargyl ether 97%

Lieferant: Thermo Fisher Scientific
Beschreibung: Appearance: Clear colorless to light yellow Liquid

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Product Image-AATB3010

N-Succinimidyl 6-Biotinamidohexanoate ≥90%

Artikel-Nr: (AATB3010)
Lieferant: AAT BIOQUEST
Beschreibung: This biotin has a spacer arm of ~22 Å that reduces steric hindrance when binding several biotinylated molecules to one avidin complex; This reagent penetrates cell membranes because there is no charged group.
VE: 1 * 25 mg

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Image Unavailable-SIAL21955-25MG

5(6)-Carboxytetramethylrhodamine succinimidyl ester [5(6)-TAMRA SE], BioReagent geeignet für Fluoreszenz, Sigma-Aldrich®

Artikel-Nr: (SIAL21955-25MG)
Lieferant: Merck
Beschreibung: 5(6)-Carboxytetramethylrhodamine N-succinimidyl ester (TMR-SE) is used as an amine coupling reagent to form 5(6)-carboxytetramethylrhodamine (TMR) derivatized compounds such as proteins and drugs.
VE: 1 * 25 mg
Product Image-PIER22298

BMPS (3-Maleimidopropionic acid N-hydroxysuccinimide ester), Pierce™

Artikel-Nr: (PIER22298)
Lieferant: Thermo Fisher Scientific
Beschreibung: Thermo Scientific Pierce BMPS is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a short spacer arm (5.9 Å).
VE: 1 * 50 mg
Product Image-PIER26169

SDAD (NHS-SS-Diazirine) (succinimidyl 2-((4,4'-azipentanamido)ethyl)-1,3'-dithiopropionate), Pierce™

Artikel-Nr: (PIER26169)
Lieferant: Thermo Fisher Scientific
Beschreibung: Thermo Scientific Pierce SDAD (NHS-SS-Diazirine) combines proven NHS-ester and diazirine-based photoreaction chemistries with conjugate amine-containing molecules with nearly any other functional group via long-wave UV-light activation. A 13.5Å spacer arm containing a cleavable disulfide bond separates the two photoreactive groups.
VE: 1 * 50 mg
Image Unavailable-AATB21242

Protonex™ Lyso-NIR 770

Artikel-Nr: (AATB21242)
Lieferant: AAT BIOQUEST
Beschreibung: Protonex™ Lyso-NIR 770 is a fluorescent derivative prepared from our Protonex™ NIR 770 NHS ester.
VE: 1 * 1 mg

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Image Unavailable-SIAL803626-50MG

SMPH (Succinimidyl-6-((beta-maleimidopropionamido)hexanoate) ≥90%, Sigma-Aldrich®

Artikel-Nr: (SIAL803626-50MG)
Lieferant: Merck
Beschreibung: SMPH is a heterobifunctional crosslinker with N-hydroxysuccinimide (NHS) ester and maleimide groups that allow covalent conjugation of amine- and sulfhydryl-containing molecules. NHS esters react with primary amines at pH 7-9 to form amide bonds, while the maleimides react with sulfhydryl groups at pH 6.5-7.5 to form stable thioether bonds. In aqueous solutions, hydrolytic degradation of the NHS ester is a competing reaction whose rate increases with pH. The maleimide group is more stable than the NHS-ester group but will slowly hydrolyze and also lose its reaction specificity for sulfhydryls at pH values greater than 7.5. For these reasons, conjugation experiments involving this type of heterobifunctional crosslinker are usually performed at pH 7.2-7.5, with the NHS-ester (amine-targeted) reaction being accomplished before or simultaneous with the maleimide (sulfhydryl-targeted) reaction.
VE: 1 * 50 mg
Image Unavailable-SIAL803383-50MG

LC-SMCC (N-Succinimidyl 6-[[4-(N-Maleimidomethyl)cyclohexyl]carboxamido]hexanoate) ≥90%, Sigma-Aldrich®

Artikel-Nr: (SIAL803383-50MG)
Lieferant: Merck
Beschreibung: LC-SMCC is a heterobifunctional crosslinker with N-hydroxysuccinimide (NHS) ester and maleimide groups that allow covalent conjugation of amine- and sulfhydryl-containing molecules. NHS esters react with primary amines at pH 7-9 to form amide bonds, while the maleimides react with sulfhydryl groups at pH 6.5-7.5 to form stable thioether bonds. In aqueous solutions, hydrolytic degradation of the NHS ester is a competing reaction whose rate increases with pH. The maleimide group is more stable than the NHS-ester group but will slowly hydrolyze and also lose its reaction specificity for sulfhydryls at pH values greater than 7.5. For these reasons, conjugation experiments involving this type of heterobifunctional crosslinker are usually performed at pH 7.2-7.5, with the NHS-ester (amine-targeted) reaction being accomplished before or simultaneous with the maleimide (sulfhydryl-targeted) reaction
VE: 1 * 50 mg
Product Image-AATB345

5-CR6G, SE einfaches Isomer ≥90%

Artikel-Nr: (AATB345)
Lieferant: AAT BIOQUEST
Beschreibung: 5-CR6G, SE is amine-reactive derivative of 5-CR6G.
VE: 1 * 5 mg

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Product Image-PIER22339

SBAP (Succinimidyl 3-(bromoacetamido)propionate), Pierce™

Artikel-Nr: (PIER22339)
Lieferant: Thermo Fisher Scientific
Beschreibung: Thermo Scientific Pierce SBAP is a short (6.2 angstrom) crosslinker for amine-to-sulfhydryl conjugation via N-hydroxysuccinimide (NHS) ester and bromoacetyl reactive groups.
VE: 1 * 50 mg
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